NS-200/NS-200C Nano Scan Microplate Reader 200

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Product Description

Versatile Microplate Readers for Scalable Biotech, Life Science & Diagnostic Research The Nano Scan Microplate Reader NS-200 series are next-generation microplate readers engineered for high-throughput, high-precision absorbance measurements, making it an essential analytical platform for modern biotech laboratories. Optimized for ELISA, enzyme kinetics, and nucleic acid / protein quantification, the system integrates a high-resolution grating monochromator covering 200–1000 nm with 1 nm step accuracy—supporting full-spectrum scanning for a wide range of biochemical and molecular assays.

NS-200 designed to streamline complex workflows, the NS-200 series accommodates both 96- and 384-well microplates, features programmable orbital shaking, and offers precise temperature control up to 45 °C—ideal for temperature-sensitive reactions and cell-based assays. An integrated cuvette slot further expands assay capabilities for NS-200C, enabling endpoint, kinetic, and spectral measurements without external modules. Additional quantification application can be supported through the optional Nano-plate module, available as a separate purchase.

The NS-200 series ensure exceptional data stability and repeatability—meeting the rigorous standards of biotech research, diagnostic research, food safety and agriculture, as well as assay development environments; delivers the performance, flexibility and reliability modern laboratories demand.

Highlights

  • Equipped with 10-inch capacitive touchscreen
  • Support standalone operation & PC-connectivity through ReaderIt-II software with advanced data management
  • Adopts xenon flash lamp as light source, which covers wide wavelength range 200-1000nm wavelength with 1nm increments with High-Resolution Monochromator
  • Features incubation up to 45°C and three adjustable shaking speeds
  • Compatible with 96- & 384-well microplates
  • Separated sold unit of Nano-plate – quantification of nucleic acid and protein
  • Includes an independent cuvette slot for NS-200C only
  • Uses an optical double beam system with a reference channel; paired with automatic calibration of all critical components for consistent and reliable results

Applications

  • ELISA
  • Protein quantification
  • Enzyme kinetic assay
  • Endpoint method
  • Kinetics scanning
  • Spectral scanning
  • Nucleic Acid quantification (using Nano-plate)

Technical Data

SpecificationsNano Scan microplate Reader,
NS-200, with monochromator wavelength 200-1000nm,
AC 100-240V		Nano Scan microplate Reader,
NS-200C supplied with cuvette,
with monochromator wavelength 200-1000nm, AC 100-240V
Display10 inch high-resolution capacitive touch screen
Detection system2 silicon photodetectors (measurement & reference)
Light sourceXenon flash lamp / number of flashes > 10^9
Wavelength range200 - 1000nm
Wavelength accuracy2nm
Wavelength repeatability0.2nm
Optical filterMonochromator, 1nm step
Half-bandwidth of filters<2.5nm
Dynamic range0 - 4 Abs
Compatible plate type96- & 384-well plate96- & 384-well plate; cuvette
Function96- & 384-well plateCuvette
Linearity (450nm)R2 ≥ 0.999, (0.0 - 3.0Abs)N/A
Accuracy (450nm)±(1.0% + 0.003 Abs) @ (0.0 - 2.0 Abs) ±2.0% @ (2.0 - 2.5 Abs)N/A
Precision (450nm)CV < 0.5% (Precision mode); CV < 1.0% (Fast mode)CV < 0.5%
Stability (450nm)CV < 0.5% (Precision mode); CV < 1.0% (Fast mode)< 0.005 Abs, (0.0 - 2.0 Abs) < 2%, (2.0 - 2.5 Abs)
Reading speed< 8 seconds at Fast mode (96-well plate) < 28 seconds at Precision mode (96-well plate)N/A
Reading modeEnd point, Dual Mode, Kinetic
Incubation rangeRT+ 4°C to 45°C
Temperature accuracy± 0.5°C @ 37°C
Temperature uniformity± 0.5°C @ 37°C
Shaking modeLinear, 3 speeds adjustable
User interfaceBuilt-in software, independent use
Input VoltageAC 100-240V, 50-60Hz
Storage16GB storage, can store more than 20,000 data files
Ports2 type A USB interfaces, 1 Type B USB interface, 1 ethernet port
Dimension300mm (W) x 500mm (L) x 260mm (H)
Net weight15.5kg

Resources

Documents

Data Sheet : NS-200/NS-200C Nano Scan Microplate Reader 200

Ordering Info

Cat. No.Nano Scan Microplate Reader, NS-200
NS-200Nano Scan microplate Reader NS-200, with monochromator wavelength 200-1000nm, AC 100-240V
NS-200CNano Scan microplate Reader NS-200C supplied with cuvette, with monochromator wavelength 200-1000nm, AC 100-240V

Optional Accessories

Cat. No.Accessories for Nano Scan Microplate Reader, NS-200
NS-200-NanoPNano-plate
NS-200-VBoardOptical Performance Validation Board for NS-200 series
NS-200-SIIReaderIT-II PC software of NS-200 series

FAQ

  1. What are the common combinations of ELISA conjugate/substrate and wavelengths?
Enzyme labelSubstrateWavelength
Horseradish peroxidase (HRP)TMB (3,3′,5,5′-tetramethylbenzidine)450
OPD (o-Phenylenediamine)492
ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid)405
Alkaline phosphatase (AP)pNPP ((p-Nitrophenyl Phosphate))405
BCIP (5-bromo-4-chloro-3-indoxyl phosphate)405
β-galactosidase (β-gal)ONPG ((o-Nitrophenyl-ß-D-galactopyranosidase)420
UreaseUrea/Bromocresol590
  1. What wavelength should I use for protein quantification?
Protein QuantificationWavelength
Bradford595
BCA562
Lowry650
Modified Lowry750
Biuret540
  1. Should I use a spectrophotometer or a microplate reader for protein quantification?
    Both instruments can be used. However, the limitation of a spectrophotometer is that only one sample (in a cuvette) can be measured at a time. If you have more samples and want to save time, it is advisable to use a microplate reader.
  2. Why are the results different for the same sample in different wells?
    The quality of microplates is very important as the light source passes through the sample vertically, either from top to bottom or bottom to top. Prevent from using microplates with smudges or scratches and other forms of optical interference that may obstruct the light beam. A reference wavelength should be employed for quality control. The reference wavelength can be obtained from the manual/protocol of your specific assay.
  3. Why do we need to shake our samples?
    Depending on the experiment, sometimes shaking is required to allow uniform cell suspension (e.g. cell proliferation assay). Shaking can also bring down the droplets stuck on the wall of the well for a more accurate measurement.
  4. What is the difference between end point, kinetic and dual mode?
    End point mode refers to measurement of each well only once. Kinetic mode measures each well once (like end point) but repeats the process (e.g. enzyme kinetic assay, cell migration and invasion). Dual mode uses two wavelengths to measure each well (like end point) at the same time.
  5. Should I read my ELISA plates immediately after adding the stop solution?
    It is recommended to measure the plates immediately after adding stop solution because excessive time lapse may cause the absorbance reading to shift.
  6. Why do I get low readings for ELISA?
    This may be caused by several factors such as very low laboratory temperature (optimal working temperature is 18-25°C), incubation periods too short, reagents and buffers too cold, contaminated washer and wrong conjugates being used.
  7. The standard filters are not suitable for my research.
    We have a total of 28 filters with various wavelengths to accommodate most applications. Kindly check the Ordering Information.
  8. Should we turn off the microplate reader when not in use?
    Yes. We recommend turning off your microplate reader at the end of the day or when not in use for long period of time. This can extend the shelf life of tungsten halogen lamp as well as initiate a diagnostic self-check.

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