The 2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to measure the scavenging ability of both tamarind fruit pulp extracts and the lotion formulations. The DPPH test was performed by using a 96-well plate and  NS-100 Nano Scan Microplate Reader from Hercuvan by a research group from Prince of Songkla University aimed to study the formulation and investigate antioxidant potential of O/W lotions containing Tamarindus indica L, Fruit pulp extract.