The DNA extracted is used as template by research group from University Putra Malaysia (UPM) for PCR amplification performed using forward and reverse primers on a GS-96 Gradient Touch Thermal Cycler (Hercuvan Lab System) in an investigation involved isolation and optimization of phenol degradation by antarctic isolate using one factor at timeThe spleens, kidneys, livers and brains were surgically collected, weighed, and homogenized in 2ml phosphate-buffered saline (PBS) by using a Hercuvan digital handheld homogenizer from a research group of Hungary to investigate the Signaling through Syk or CARD9 Mediates Species-Specific Anti-Candida Protection in Bone Marrow Chimeric Mice.